effect of non-fiber carbohydrates on in vitro first order kinetics disappearance of cellulose

an in vitro experiment was conducted to determine the effect of supplemental non-fiber carbohydrate(nfc) on the disappearance kinetics of cellulose (ce) by mixed ruminal microorganisms. non-supplementedor nfc supplemented cellulose (467 mg nfc/g cellulose as sucrose (cesu) or starch (cest) or a 1:1 mixtureof sucrose + starch (cesust)) were incubated for 24, 48, and 96 h at 39°c. after each incubation time, ph,ammonia-n concentration and cellulose disappearance were measured. the disappearance kinetics rate of all samples was determined using first order exponential model of d(t) = d(i).exp (-k. time) + i; where d(t) is potentially digestible fraction; d(i) is potentially digestible residues; k is digestion rate of cellulose (h-1) and i is indigestible fraction. in this experiment, inclusion of nfc to the rumen fluid medium resulted in asignificant depression (p